Folic Acid Biotechnological Production and Applications

Folic acrimonious Biotechnological Production and ApplicationsNUR SHAHIRAH BINTI BAHARUDINSummaryFolic bitter (synthetic form) which too known as folate (naturally occurring form) is a extremity of the water soluble B complex vitamin. Folic sexually transmitted illness is man-made. It whoremonger be found in many beans, grains, fortified breakfast cereals, pasta, and green ve payables. Folic dot plays self-aggrandising part in building DNA and aids the body to eviscerate new new cells, especially red agate line cells. Folic window pane hold common properties of water-soluble vitamin which are enter the bloodstream properties immediately after being consumed through excretion in urine. Other than that, unlike fat-soluble vitamin, they are easily done for(p) in heat or by air pressure. These folic acid are absorbed into blood via portal vein to the liver.Vitamin B9 including folic acid and folate is grievous for some(prenominal) bodily functions which are used for synt hesizing and repairing DNA, RNA and , assisting rapid cell division and growth to produce healthy red blood cells. It is important for pregnant women to thrust enough folic acid to prevent major birth defects of her cockers brain or spine and enhances brain health. The memory is said to be improved through folic acid consumption. The human body does not synthesize folic acid, at that placefore to make sure we have adequate amounts ,we should take it everyday. To reduce the number of unquiet tube defects there are many countries are adopted the policy of fortification of folic acid. When fortification is introduced , many people are exposed to an increased aspiration of folic acid .With this, skittish tube defects can be prevented. For animals, diet that consists can influence DNA and histone. This races to phenotypic changes in next generation. While for humans, the increased intake leads to elevated blood concentrations and of unmetabolized folic acid. This related to decreas ed natural killer cell cytotoxicity, and reduce the response to antifolate drugs used against cancer, malaria, and cancer.Keywords Folic acid, water-soluble vitamin,fat-soluble vitamin, methylation,antifolate1 INTRODUCTIONHistory of Folic acidThrough a research made by Indian scientist, there is connection between the macrocytic anaemia and lack in the diet. However, what kind of deficiency was not determined.The name of folic acid was suggested which coming from the Latin word for the leaf foium,due to a factor suspected of having vitamin-like properties in animals that was isolated from spinach.In 1945, the importance of folic acid to treat a sign of amenia during pregnancy can be seen through a demonstration. In 1962 , Dr. Victor Herbert conducted experiment on himself in order to show a deficit of folic acid hitd a type of anemia.The experiment is successful. It was Dr. Herberts findings that set the criteria for the diagnosis of folate deficiency and the folic acid adult r equirement.Figure 1.1 Chemical Structure of Folic Acid2 FOLIC ACID BIOLOGICAL ROLES2.1 DNA and Cell DivisionFor the production DNA and RNA synthesis and maintaining new cells, folate is required. And it is also required in preventing changes to DNA, and, and so, for preventing cancer. More specifically it helps in the manufacture of nucleic acids like Thymine. A folate coenzyme is required for the synthesis of methionine, and methionine is required for the synthesis of S-adenosylmethionine (SAM). SAM is a methyl assembly (one-carbon unit) donor used in many biological methylation reactions, including the methylation of a number of sites within DNA and RNA.2.2 Biochemistry of DNA base and amino acid productionIn the form of a series of tetrahydrofolate (THF) compounds, folate derivatives are substrates in a number of single-carbon-transfer reactions, and also are involved in the synthesis of dTMP (2-deoxythymidine-5-phosphate) from dUMP (2-deoxyuridine-5-phosphate). It is a substr ate for an important reaction that involves vitamin B12 and it is necessary for the synthesis of DNA and required for all dividing cells.2.3 Conversion to biologically active derivativesAll the biological functions of folic acid are performed by tetrahydrofolate and other derivatives. Their biological availability to the body depends upon dihydrofolate reductase action in the liver. This action is un usually slow in humans. Due to this low activity, it has been suggested this limits the conversion of folic acid into its biologically active forms when folic acid is consumed at levels higher than the Tolerable Upper Intake Level (1 mg/d for adults)3 FOLIC ACID USES AND EFFECTIVENESSKidney diseaseAbout 90% of people with serious kidney disease have high levels of homocysteine. High levels of homocysteine have been linked to heart disease and stroke.By taking folic acid,it may deject homocysteine levels in people with serious kidney disease. However,through a research, folic acid supp lementation are found fail to reduce the risk of heart disease-related events.Colon CancerFolic acid play big role of protection for patients with the inflammatory bowel condition ulcerative colitis are particularly at risk for colon cancer. In a subsequent study, researchers examined the effects of folate on a laboratory model of colorectal cancer development in mice. They discovered that animals consuming a folate-deficient diet developed intestinal masses, while those mice on a control diet consuming adequate amounts of folate remained free of tumors. The conclusion is that folate deficiency increased DNA dam come on by decreasing the expression of two genes involved in DNA repair. This study is consistent with the majority of epidemiological studies in humans, which demonstrate a clear link between colorectal cancer development and inadequate folate consumption.4 Folic Acid The most essential wholesome for PregnancyFolic acid is well-known as the most essential nutrient for pr egnancy. There are many studies have shown that women can reduce the risk that their baby will be born with a serious neural tube defect ( incomplete development of the brain and spinal anaesthesia cord) up to 70% for women who get 400 micrograms (0.4 milligrams) daily prior to conception and during early pregnancy.The most common neural tube defects are spina bifida, an incomplete closure of the spinal cord and spinal column anencephaly, operose underdevelopment of the brain encephalocele, when brain tissue protrudes out to the skin from an abnormal opening in the skull.Its so important for all women of childbearing age to get enough folic acid , not just those who are planning to become pregnant, be case all of these defects occur during the first 28 days of pregnancy usually before a woman even knows shes pregnant. Through a survey, only 50% of pregnancies are planned, so any woman who could become pregnant should make sure shes getting enough folic acid.Folic acid has no use bi ochemically in the body until it is converted into these other forms of folate such asTetrahydrofolate (THF)5-formyltetrahydrofolate (Folinic acid)5-10-methylenetetrahydrofolate5-methyltetrahydrofolate (Methylfolate5 FOOD SOURCES OF FOLATEFolic acid is a form of folate. Since folate is water-soluble B vitamin, it is found naturally in foods. This vitamin cannot be synthesized in our body. Folate is important for everyone especially it helps make red blood cells. If you do not have enough folate, you can get a type of anaemia. If you have anaemia you may feel tired, weak and unable to concentrate. Folate also very important for infants, children and women who are pregnant. It helps to prevent some birth defects. Women who could become pregnant, are pregnant or breastfeeding need more folate. They should take a daily folic acid supplement of 400 mcg (0.4 mg).In addition to poor dietary intake of deficient intake of other B vitamins can contribute to folate deficiency. These vitamins i nclude B1, B2, and B3 which are all involved in folate recycling. Poor protein intake can cause deficiency of folate binding protein which is needed for optimal absorption of folate from the intestine, and can also be related to an insufficient supply of glycine and serine, the amino acids that directly participate in metabolic recycling of folate. Excessive intake of alcohol, smoking, and heavy coffee drinking can also contribute to folate deficiency.How frequently folate should everyone aim?Nutrient InteractionsHow do other nutrients interact with folate ?Vitamins B1, B2, and B3 must be present in adequate amounts to enable folic acid to can hide a vitamin B12 deficiency, by masking blood-related symptoms.A Food Rating System is created in order to better help to identify foods that give birth a high concentration of nutrients for the calories they contain, we created This system allows us to highlight the foods that are especially rich in particular nutrients. The following cha rt shows the Worlds Healthiest Foods that are both an excellent, very good, or good source of folate. Next to each food name, we may find the serving size we used to calculate the foods nutrient compositionTable 1.2 Use the following to determine the intake of folate6 FOLIC ACID DEFICIENCYShortage of folic acid can cause severe health problems in military issue, including anaemia, cardiovascular and liver diseases. A study out today revealed that the metabolism of folic acid not only impacts the immediate offspring but can also have detrimental health effects on the next several generations due to a mutation in a gene necessary. The new research, which also focus on the molecular(a) mechanism of folic acid.The damaging effects of folic acid deficiency on development are sort of renowned. This is demonstrated t by the researchers, from the Universities of Cambridge and Calgary. They used mice in this experiment as they metabolize folic acid very similarly to humans, because folic acid deficiency or mutations in the same genes required to break down folic acid in humans result in similar developmental abnormalities and diseases in mice. This enables the researchers to analyze the effects of how the molecular mechanism of folic acid deficiency impacted development that causing health problems.Other than that, inadequate in either B12 or folate can lead to macrocytic anaemia, a disease which red blood cell are larger than normal. It characterized by the production of fewer but larger red blood cells, thus a reduce ability to carry oxygen. Those affected may be weak, light-headed, and short of breath due to anemia. A deficiency in B12 can also result in varying degrees of neuropathy or nerve damage .The symptoms of lack of B12 can cause itchy and numbness in the persons hands and feet. In severe cases, mental changes that range from confusion and irritability to dementia may appear.For proper foetal development, pregnant women need sufficient amounts of folate.I ncreased amounts of folate required because of the added stress of chop-chop growing cells (the foetus), If a woman has a folate deficiency during pregnancy, it will be quicken during gestation and may lead to premature birth and neural tube birth defects, such as spina bifida, in the child. The number of neural tube defect cases decreased by 36% in the U.S. since focusing on folate supplements during pregnancy. Restless leg syndrome during pregnancy is another neurologic symptom associated with decreased folate.